Small-subunit rRNA genes and in situ hybridization with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of "Candidatus endobugula sertula".
نویسندگان
چکیده
Larvae of the bryozoan Bugula neritina harbor bacterial symbionts. These symbionts were identified as a novel species of gamma-proteobacterium, based on ribosomal small-subunit rRNA gene sequences. In situ hybridization with oligonucleotides specific for the symbiont confirmed the origin of the sequence. The taxonomic status "Candidatus Endobugula sertula" is proposed for the larval symbiont.
منابع مشابه
Differential Gene Expression in Bugula Neritina during Symbiotic Association with "Candidatus Endobugula Sertula"
The colonial marine bryozoan, Bugula neritina, harbors an uncultured endosymbiont, “Candidatus Endobugula sertula” throughout its life stages. The bacterial symbiont has been proposed to be a source of complex polyketide metabolites, the bryostatins, that chemically defend B. neritina larvae from predation. Within a bryozoan colony, significantly higher amounts of bryostatins are found in ovice...
متن کامل"Candidatus Endobugula glebosa," a specific bacterial symbiont of the marine bryozoan Bugula simplex.
The bryozoans Bugula neritina and Bugula simplex harbor bacteria in the pallial sinuses of their larvae as seen by electron microscopy. In B. neritina, the bacterial symbiont has been characterized as a gamma-proteobacterium, "Candidatus Endobugula sertula." "Candidatus E. sertula" has been implicated as the source of the bryostatins, polyketides that provide chemical defense to the host and ar...
متن کاملEvidence for the biosynthesis of bryostatins by the bacterial symbiont "Candidatus Endobugula sertula" of the bryozoan Bugula neritina.
The marine bryozoan, Bugula neritina, is the source of the bryostatins, a family of macrocyclic lactones with anticancer activity. Bryostatins have long been suspected to be bacterial products. B. neritina harbors the uncultivated gamma proteobacterial symbiont "Candidatus Endobugula sertula." In this work several lines of evidence are presented that show that the symbiont is the most likely so...
متن کاملIsolation of two polyketide synthase gene fragments from the uncultured microbial symbiont of the marine bryozoan Bugula neritina.
"Candidatus Endobugula sertula," the uncultured microbial symbiont of the bryozoan Bugula neritina, produces ecologically and biomedically important polyketide metabolites called bryostatins. We isolated two gene fragments from B. neritina larvae that have high levels of similarity to polyketide synthase genes. These gene fragments are clearly associated with the symbiont and not with the host.
متن کاملOntogeny of a symbiont-produced chemical defense in Bugula neritina (Bryozoa)
The larvae of the arborescent bryozoan Bugula neritina are chemically defended against predators by unpalatable secondary metabolites called bryostatins. Bryostatins, polyketidederived secondary compounds, are produced by the bacterial symbiont ‘Candidatus Endobugula sertula’, which is present throughout all life stages of B. neritina. Crude extracts of larval and all juvenile stages of B. neri...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 63 11 شماره
صفحات -
تاریخ انتشار 1997